Free Radical Biomedicine: Principles, Clinical Correlations, and Methodologies

Detection of Damage of Biomolecules by Free Radicals and Related Reactive Species

Author(s): Y. Robert Li

Pp: 295-306 (12)

DOI: 10.2174/978160805322311201010295

* (Excluding Mailing and Handling)

Abstract

Lipids, proteins, and nucleic acids are major cellular targets of reactive oxygen and nitrogen species (ROS/RNS). Oxidative damage to these cellular constituents has been increasingly recognized as a significant pathophysiological event leading to many disease processes. Thus, determination of ROS/RNSelicited damage to lipids, proteins, and nuclear acids is crucial for investigating the oxidative mechanisms of human diseases and assessing the efficacy of antioxidant-based therapies. This chapter first introduces the general experimental approaches to assessing the effects of ROS/RNS on cellular biomolecules and then describes the commonly used methods to determine ROS/RNS-mediated biological damage, with an emphasis on the assays that detect lipid peroxidation, protein oxidation, and oxidative DNA base modifications. The chapter ends with a discussion of biomarkers of oxidative stress and their value in assessing disease pathophysiology as well as the efficacy of antioxidant intervention.


Keywords: Biomarkers, DNA base modifications, DNA damage, F2-isoprostanes, Lipid peroxidation, Methodologies, Oxidative stress, Protein oxidation, Reactive nitrogen species, Reactive oxygen species.

Related Journals
Related Books
© 2024 Bentham Science Publishers | Privacy Policy