Generic placeholder image

Combinatorial Chemistry & High Throughput Screening

Editor-in-Chief

ISSN (Print): 1386-2073
ISSN (Online): 1875-5402

A Sensitive Voltammetric Sensor for Detecting Betamethasone in Biological Fluids

Author(s): Rajendra N. Goyal, Sunita Bishnoi and Anoop Raj S. Rana

Volume 13, Issue 7, 2010

Page: [610 - 618] Pages: 9

DOI: 10.2174/1386207311004070610

Price: $65

Abstract

A rapid and sensitive voltammetric sensor based on reduction of betamethasone has been developed using single wall carbon nanotube modified edge plane pyrolytic graphite electrode (SWNT/EPPGE). The reduction of betamethasone occurred in a well-defined, pH dependent peak. Linear calibration curve was obtained in the range 1 to 25 nM in 1.0 M phosphate buffer solution (PBS) of pH 7.2 with the limit of detection (3/slope) as 0.50 nM. The analytical utility of the developed method has been demonstarted by sensing the drug in human body fluids and for the determination of betamethasone content in several commercially available pharmaceutical preparations. Interfering effect of some common metabolites including ascorbic acid, uric acid, albumin and hypoxanthine has also been evaluated. A comparison of the observed results of proposed method with HPLC clearly indicates that the results of both methods are essentially similar.

Keywords: Betamethasone, voltammetry, carbon nanotubes, doping, biological fluids


Rights & Permissions Print Cite
© 2024 Bentham Science Publishers | Privacy Policy