Abstract
Gene therapy offers a novel approach for the prevention and treatment of a variety of diseases, but it is not yet a common method in clinical cases because of various problems. Viral vectors show high efficiency of gene transfer, but they have some problems with toxicity and immunity. On the other hand, plasmid deoxyribonucleic acid (DNA)-based gene transfer is very safe, but its efficiency is relatively low. Especially, plasmid DNA gene therapy is used for cardiovascular disease because plasmid DNA transfer is possible for cardiac or skeletal muscle. Clinical angiogenic gene therapy using plasmid DNA gene transfer has been attempted in patients with peripheral artery disease, but a phase III clinical trial did not show sufficient efficiency. In this situation, more efficient plasmid DNA gene transfer is needed all over the world. This review focuses on plasmid DNA gene transfer and its enhancement, including ultrasound with microbubbles, electroporation, hydrodynamic method, gene gun, jet injection, cationic lipids and cationic polymers.
Keywords: Plasmid DNA, peripheral artery disease (PAD), ultrasound, electroporation, hydrodynamic method, ultrasound, microbubble, cavitation, cavitation, Gene Transfer
Current Topics in Medicinal Chemistry
Title:Therapeutic Option of Plasmid-DNA Based Gene Transfer
Volume: 12 Issue: 15
Author(s): Yoshiaki Taniyama, Junya Azuma, Yasuo Kunugiza, Kazuma Iekushi, Hiromi Rakugi and Ryuichi Morishita
Affiliation:
Keywords: Plasmid DNA, peripheral artery disease (PAD), ultrasound, electroporation, hydrodynamic method, ultrasound, microbubble, cavitation, cavitation, Gene Transfer
Abstract: Gene therapy offers a novel approach for the prevention and treatment of a variety of diseases, but it is not yet a common method in clinical cases because of various problems. Viral vectors show high efficiency of gene transfer, but they have some problems with toxicity and immunity. On the other hand, plasmid deoxyribonucleic acid (DNA)-based gene transfer is very safe, but its efficiency is relatively low. Especially, plasmid DNA gene therapy is used for cardiovascular disease because plasmid DNA transfer is possible for cardiac or skeletal muscle. Clinical angiogenic gene therapy using plasmid DNA gene transfer has been attempted in patients with peripheral artery disease, but a phase III clinical trial did not show sufficient efficiency. In this situation, more efficient plasmid DNA gene transfer is needed all over the world. This review focuses on plasmid DNA gene transfer and its enhancement, including ultrasound with microbubbles, electroporation, hydrodynamic method, gene gun, jet injection, cationic lipids and cationic polymers.
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Taniyama Yoshiaki, Azuma Junya, Kunugiza Yasuo, Iekushi Kazuma, Rakugi Hiromi and Morishita Ryuichi, Therapeutic Option of Plasmid-DNA Based Gene Transfer, Current Topics in Medicinal Chemistry 2012; 12 (15) . https://dx.doi.org/10.2174/156802612803531342
DOI https://dx.doi.org/10.2174/156802612803531342 |
Print ISSN 1568-0266 |
Publisher Name Bentham Science Publisher |
Online ISSN 1873-4294 |
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